To determine the tissue specific expression profile of bis, we carried out Northern blot analysis. As shown in Figure 3a, bis gene was expressed as a single mRNA species of kb in a variety of adult human tissues, with high levels in skeletal muscle, heart and ovary. Expression of bis during mouse embryogenesis was also studied by Western blotting using an anti human Bis antibody which cross reacted with mBis, revealing that Bis was present in embryos as early as 10.5 days of gestation and that the amount of Bis increased towards birth (Figure 3b)..

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